{"id":6145,"date":"2023-12-04T16:35:00","date_gmt":"2023-12-04T15:35:00","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/sorensens-phosphate-buffer\/"},"modified":"2024-05-17T14:58:18","modified_gmt":"2024-05-17T12:58:18","slug":"sorensens-phosphate-buffer","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/pt-pt\/produto\/sorensens-phosphate-buffer\/","title":{"rendered":"Sorensen’s Phosphate Buffer"},"content":{"rendered":"

Product description :<\/strong><\/h4>\n

Sorensen phosphate buffer, also known as mixed sodium phosphate salts, is widely used in biology. The osmolarity of a 0.1 M solution at pH 7.2 is 226 milliosmoles; with the addition of 0.18 M sucrose to thesolution, the osmolarity rises to 425 milliosmoles. pH can be specified when ordering. The maximum shelf life after receipt is 3 months. All products used are chosen for their purity. Storage: 4-8\u00b0C. Sorensen phosphate buffer is available in different pH ranges: 5.8 – 8.<\/p>\n

Application :<\/strong><\/h4>\n

Sorensen phosphate buffer serves as a physiologically relevant buffer widely utilized in various experiments. It closely mimics certain components found in extracellular fluids, rendering it non-toxic to cells. The buffer demonstrates minimal pH fluctuations with temperature shifts and remains stable for several weeks when stored at 4\u00b0C. However, it is prone to precipitation during fixation and tends to form precipitates with uranium acetate and react with lead salts. Additionally, it slowly contaminates with microorganisms.<\/p>\n

Tip :<\/h4>\n

For optimal preparation, phosphate buffer solutions should be stored at 4-8\u00b0C, and the shelf life after opening is 3 months.<\/p>\n

Additional Information :<\/strong><\/h4>\n

Mother Solutions:<\/em><\/p>\n

    \n
  • Sodium dibasic phosphate 0.2 M 1 liter<\/li>\n
  • Sodium monobasic phosphate 0.2 M 1 liter<\/li>\n<\/ul>\n

    Working Buffer: <\/em>Prepare a 0.1 M working buffer using 100 ml of Sorensen phosphate buffer. Mix X ml of sodium dibasic phosphate 0.2 M with Y ml of sodium monobasic phosphate. Dilute to 100 ml with ddH20 or dilute 1:1 with fixative.<\/p>\n

    pH (25\u00b0C) X ml Y ml<\/p>\n\n\n\n\n\n\n\n\n\n\n
    pH<\/th>\nX ml<\/th>\nY ml<\/th>\n<\/tr>\n<\/thead>\n
    7.0<\/td>\n30.5<\/td>\n19.5<\/td>\n<\/tr>\n
    7.2<\/td>\n36.0<\/td>\n14.0<\/td>\n<\/tr>\n
    7.4<\/td>\n40.5<\/td>\n9.5<\/td>\n<\/tr>\n
    7.6<\/td>\n43.5<\/td>\n6.5<\/td>\n<\/tr>\n
    7.8<\/td>\n45.75<\/td>\n4.25<\/td>\n<\/tr>\n
    8.0<\/td>\n47.35<\/td>\n2.65<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    Osmolarity varies by adjusting the phosphate molarity or by adding sucrose, glucose, or sodium chloride. At pH 7.2:<\/p>\n

      \n
    • 0.10 M = 226 mOs (milliosmoles)<\/li>\n
    • 0.05 M = 118 mOs<\/li>\n
    • 0.075 = 180 mOs<\/li>\n
    • 0.15 M = 350 mOs<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"
        \n
      • available from pH 6.9 to 7.4<\/li>\n
      • production on demand<\/li>\n
      • microfiltered 0.22\u00b5 – st\u00e9rile<\/li>\n
      • sealed under neutral gas (Argon)<\/li>\n
      • made in France<\/span><\/span><\/span><\/span><\/li>\n<\/ul>\n","protected":false},"featured_media":6147,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[402],"product_tag":[],"class_list":["post-6145","product","type-product","status-publish","has-post-thumbnail","product_cat-tampao","pa_buffer-concentration-015m-pt-pt","pa_buffer-concentration-01m-pt-pt","pa_buffer-concentration-02m-pt-pt","pa_volume-1000ml-pt-pt","pa_volume-125ml-pt-pt","pa_volume-250ml-pt-pt","pa_volume-500ml-pt-pt","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/product\/6145","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media\/6147"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media?parent=6145"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/product_brand?post=6145"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/product_cat?post=6145"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/product_tag?post=6145"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}