Neutral Buffered Formalin (NBF) 10%

55,35 58,69 

Phosphate Buffered Only for Histology , light Microscopy

  • not used electron microscopy fixation
  • pH 6.9 to 7.1
  • distilled water, phosphate buffer
  • store at room temperature (RT)
  • made in France
SKU: GF871070 Categorías: ,


Product Description:

Neutral-buffered formalin (NBF) is a commonly used fixative in histology for light microscopy only.
Composition: NBF is composed of formaldehyde (usually at a concentration of 10%, which corresponds to approximately 4% formaldehyde by volume) diluted with phosphate buffer to achieve a neutral pH. The phosphate buffer helps maintain the pH stability of the solution.


NBF serves as a fixative for preserving tissue samples. It is widely employed in preparing samples for light microscopy. Here are some key points about NBF:

  • Fixation: When tissue is immersed in NBF, formaldehyde reacts with proteins, creating a gel-like structure that retains cellular constituents in their in vivo relationship. Properly fixed tissue can withstand subsequent stages of tissue processing and staining.
  • Applications:
    • Histology: NBF is a popular general-purpose histological fixative used to fix tissues for microscopic examination.
    • Staining Techniques: It has been used in various staining techniques, including oil-red-O staining for differentiating preadipocyte cell lines and analyzing tissues like rabbit eyes and diabetic rat intestines.
    • Storage: NBF is suitable for both short-term fixation (useful for high-throughput laboratories) and long-term storage of tissue samples.

NBF is labeled with hazard statements related to toxicity, skin sensitivity, and carcinogenicity. Proper precautions should be taken when handling this solution.

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