Descripción
Product Description:
Neutral-buffered formalin (NBF) is a commonly used fixative in histology for light microscopy only.
Composition: NBF is composed of formaldehyde (usually at a concentration of 10%, which corresponds to approximately 4% formaldehyde by volume) diluted with phosphate buffer to achieve a neutral pH. The phosphate buffer helps maintain the pH stability of the solution.
Application:
NBF serves as a fixative for preserving tissue samples. It is widely employed in preparing samples for light microscopy. Here are some key points about NBF:
- Fixation: When tissue is immersed in NBF, formaldehyde reacts with proteins, creating a gel-like structure that retains cellular constituents in their in vivo relationship. Properly fixed tissue can withstand subsequent stages of tissue processing and staining.
- Applications:
- Histology: NBF is a popular general-purpose histological fixative used to fix tissues for microscopic examination.
- Staining Techniques: It has been used in various staining techniques, including oil-red-O staining for differentiating preadipocyte cell lines and analyzing tissues like rabbit eyes and diabetic rat intestines.
- Storage: NBF is suitable for both short-term fixation (useful for high-throughput laboratories) and long-term storage of tissue samples.
Tip:
NBF is labeled with hazard statements related to toxicity, skin sensitivity, and carcinogenicity. Proper precautions should be taken when handling this solution.
