Phosphate Buffered Saline (PBS)

Descripción

Product description:

Phosphate-buffered saline (often abbreviated as PBS) is a commonly used buffer solution in biochemistry. It is a physiological solution containing sodium chloride, disodium phosphate, monopotassium phosphate, and a small amount of potassium chloride. Generally, the concentration of these salts is equivalent to that of the human body (isotonicity).

Application:

This buffer is primarily used for rinsing cells to remove any traces of medium before treatment. It is intended for use on cells outside of the CO2 incubator and is not designed for long-term incubations.

The composition of 1X PBS is:

• 8.0 g/L NaCl,
• 0.2 g/L KCl,
• 1.42 g/L Na2HPO4,
• 0.24 g/L KH2PO4 for 1000 ml of distilled water.

Tip:

A buffer solution maintains approximately the same pH despite the addition of small amounts of an acid or base, or despite  dilution.

Its buffering capacity relies on the dihydrogen phosphate/hydrogen phosphate pair (pKa = 7.2), which is also one of the three major mechanisms for maintaining blood pH (HPO4^2- + H+ = H2PO4^-).
Storage: RT, Note: It crystallizes at low temperatures.