Description
MOPS is the common name for 3-morpholino-1-propanesulfonic acid. Its chemical structure features a morpholine ring, and its pKa of 7.15 at 20°C is of particular interest for biochemical applications.
MOPS is used as a buffer in biology and biochemistry. The pH of a solution prepared with MOPS depends on concentration and temperature, an effect that can be predicted and calculated. MOPS is highly soluble in water.
It is a buffer with several characteristics:
it is highly soluble,
its pKa makes it suitable for use in biology,
it is chemically stable and not subject to enzymatic reactions,
has negligible absorption in the visible and near-UV range, and is relatively easy to synthesize.
Use at concentrations above 20 mM is not recommended for mammalian cell culture.
Ref:
role of buffer in the fixation of biological specimen for electron microscopy role of buffer in the fixation of biological specimen for electron microscopy
