MOPS

23,31 

3-morpholino-1-propanesulfonic acid
Preparation of MOPS Buffer
Formula:C7H15NO4S
PM , MW : 209.26
CAS No. [1132-61-2]

Artikelnummer: GP20-25 Kategorie:

Beschreibung

MOPS is the common name for 3-morpholino-1-propanesulfonic acid. Its chemical structure features a morpholine ring, and its pKa of 7.15 at 20°C is of particular interest for biochemical applications.

MOPS is used as a buffer in biology and biochemistry. The pH of a solution prepared with MOPS depends on concentration and temperature, an effect that can be predicted and calculated. MOPS is highly soluble in water.

It is a buffer with several characteristics:

it is highly soluble,
its pKa makes it suitable for use in biology,
it is chemically stable and not subject to enzymatic reactions,
has negligible absorption in the visible and near-UV range, and is relatively easy to synthesize.
Use at concentrations above 20 mM is not recommended for mammalian cell culture.

Ref:
role of buffer in the fixation of biological specimen for electron microscopy role of buffer in the fixation of biological specimen for electron microscopy

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