Description
Sodium Phosphate, Monobasic (NaH₂PO₄·H₂O)
Sodium Phosphate, Dibasic (Na₂HPO₄·H₂O)
0.5% Sodium Chloride (NaCl)
The pH of this buffer is 7.4; its osmolarity is 440 mOsm, making this buffer hypertonic relative to most bodily fluids.
Millonig recommends using this buffer for fixing highly hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is advised.
References: Millonig (1964); Karlsson & Schultz (1965).
