STAIN77 – EMstain solution

44,10 136,50 

  • ready to use
  • for positive and negative contrast
  • pH close to 7
  • sealed under inert gas
  • made in France
SKU: GC301078 Categoría:

Descripción

Product description :

Stain77 is an extremely low-toxicity contrast agent, consisting of lithium and tungsten salts (refer to MSDS). It effectively enhances both positive and negative contrast. It remains insensitive to light and allows for long-term storage at room temperature (RT). If crystallization occurs over time, you can restore the product’s contrast-enhancing properties through filtration or centrifugation.

Application :

Stain77 serves as a genuine alternative to uranyl acetate for section contrast and negative staining, boasting a pH near 7 and ensuring user safety. Contrast results, after enhancing with lead citrate, match or even surpass those of uranyl acetate.

The Stain77 Ultra-Thin Section Contrast Protocol involves:

  1. Applying a drop of Stain77 for 1 to 2 minutes.
  2. Drying.
  3. Briefly rinsing with distilled water.
  4. Drying.
  5. Counterstaining with lead citrate for 1 minute, followed by rinsing with distilled water and drying.
  6. Observation using a Transmission Electron Microscope (TEM).

Please note: Avoid pre-storing rinse water in the refrigerator, as cold water may absorb CO2, potentially contaminating your sections.

The protocol above enhances the contrast of ultra-thin sections (positive contrast) and is also suitable for negative staining. However, negative staining does not require counterstaining with lead citrate after using Stain77.

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Tips :

It’s available in two variants and two volumes (30ml and 200ml):

  • A 30ml Airless bottle with a drop-by-drop dispenser for manual staining.
  • A 200ml option for use with the Leica EM AC20 or RMC automated contrast systems (briefly rinse with distilled water for 2×5 minutes).

Stain77 can be stored in the laboratory at room temperature and is not affected by exposure to light.

 

 

Información adicional

Volume

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