Sodium Cacodylate Buffer: An Effective Alternative to Phosphate Buffers
Did you know? Sodium cacodylate buffer (Na(CH₃)₂AsO₂·3H₂O) is one of the top choices for electron microscopy and prepping biological samples, especially when you want to skip all that extra phosphate!
Why Choose Sodium Cacodylate Buffer?
It’s awesome at keeping pH stable—especially between 5.0 and 7.4—which makes your lab work easier and more reliable.
What Makes It Better Than Phosphate Buffers?
Back in 1962, Sabatini and his team started using sodium cacodylate for EM. Why? Because adding more phosphates (like in Sørensen buffer) can mess up your samples—damaging mitochondria and other organelles. Cacodylate avoids all that, making it gentler and safer for sensitive bio samples.
Perfect Partner with Fixatives
Unlike Tris and other amine buffers, sodium cacodylate doesn’t react with aldehyde fixatives. That means your samples come out just the way you want, every time.
Anything Unique About Sodium Cacodylate?
Some say its effectiveness might actually come from how it inhibits arsenate metabolism—more than from its buffering power alone!
Want to Know More? 💡
Check out our FAQ page for answers to your questions about sodium cacodylate and other lab buffers.
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