Osmium tetroxide in Buffer

174,29 189,54 

  • ready to use (with buffer)
  • ultrapure osmium tetroxide
  • sealed under inert Argon gas in a brown glass vial
  • handle under a fume hood
SKU: GF300000 Categories: ,

Description

Product description :

Osmium Tetroxide (OsO4) is recognized for its role as a secondary fixative in electron microscopy, attributed to its slow tissue penetration and limited ability to cross-link proteins and carbohydrates. Its primary value lies in the ability to cross-link lipids, making it indispensable for preserving the intricate details of lipid-rich structures within samples.

Application :

Despite its secondary fixative status, OsO4 is crucial for its unique ability to enhance electron density. This property provides a scaffold for further staining with lead, significantly enhancing contrast and enabling the detailed observation of biological specimens under the electron microscope. It specifically targets unsaturated fatty acids and reacts with a variety of protein side chains, including thiol, hydroxyl, phenolic, carboxyl, and amino groups, with cyteine and methionine being the most reactive. This broad reactivity spectrum allows OsO4 to play a vital role in the visualization of both lipid and protein components of cells.

Tips :

Given its reactive nature, handling OsO4 requires careful consideration of safety protocols to avoid exposure risks. When utilizing OsO4, ensure that the process is conducted in a well-ventilated area or fume hood, with appropriate personal protective equipment (PPE) such as gloves and eye protection. Due to its poor tissue penetration, it is often used in conjunction with other fixatives that cross-link proteins and carbohydrates more effectively, optimizing tissue preservation and imaging quality.

  • Store in a well-sealed box at 4 – 8°C to ensure the chemical’s stability and longevity.
  • Always handle under a fume hood to protect yourself from potential exposure to harmful vapors.
  • Be cautious of very toxic vapors; proper laboratory safety protocols must be followed to prevent inhalation or contact with the skin and eyes.

 

Video tutorial on using osmium tetroxide solution vials :

 

Additional information

Osmium concentration

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Buffer type

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Volume

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