Protocole
The preparation of the OTE solution is similar to the infusion of tea water 70°C not more.
– Using a heating magnetic stirrer set at 70°C, dissolve your OTE powder at a concentration of 0.2% not more (because saturation is reached at 0.3%)
– After complete dissolution (reached in 15 to 30 minutes with stirring at 70 ° C),
- Filter your OTE extract solution at 0.45µ or better at 0.22µ
- Store in a small Eppendorf tube filled to the maximum to reduce air containing oxygen or in an AirLess vial.
- Store in the darkness at 4 °C (when handling always let your solutions return to RT)
- Contrast your grids with OTE for 15 to 20 minutes, darkness, RT
- Drain simply not rinse or very short wash 1 to 2 seconds, after drying
- Post-stain 1 to 2 minutes with lead citrate according to Reynolds (Ref # 11300)
- Drain, short rinse, dry and ready to observe.
Powder OTE , 10gr only by EM-grade.com
